Role of tyrosine kinase in serotonin-induced constriction of the basilar artery in vivo

Abstract

Background and Purpose - Serotonin is one of the most potent constrictors of cerebral blood vessels and is implicated in several pathological conditions, including migraine and cerebral ischemia. Recent evidence has suggested that tyrosine kinase is involved in vasocontractile responses. The objective of this study was to test the hypothesis that activation of tyrosine kinase contributes to serotonin-induced constriction of the basilar artery in vivo. Methods - Using a cranial window in anesthetized Sprague-Dawley rats, we examined effects of inhibitors of tyrosine kinase and tyrosine phosphatase on constrictor responses of the basilar artery to serotonin in vivo. Results - Serotonin (10-8, 10-7, and 10-6 mol/L) produced constriction of the basilar artery by 12 ± 2%, 27 ± 2%, and 37 ± 3%, respectively. Genistein (3x10-6 mol/L), an inhibitor of tyrosine kinase, did not affect baseline diameter of the basilar artery but attenuated serotonin-induced vasoconstriction (P