Magnetic Resonance Imaging in Drug Development

Abstract

Using a popular diagnostic method such as magnetic resonance imaging (MRI) for imaging cell cultures can contribute to scientific research. With an optimally selected testing protocol, it will be possible to image cell lines using MRI at the cellular level and then implement pharmaceutical substances into ba-days and test their effectiveness in cell cultures. Preliminary studies on pharmaceutical substances show that the application of MRI for in vitro experimental studies yields satisfactory differences in signal intensity, so it turns out to be important to implement in vitro studies and then try to transfer them to in vivo studies. Trastuzumab is an IgG1 monoclonal antibody that binds selectively to human epidermal growth factor receptor 2. It is used to treat breast cancer in patients whose tumors overexpress the Her-2 receptor. The implementation of cell culture research is helping to understand cell biology. Pharmaceutical research using 3D cell culture provides biologically relevant models for determining drug activity in tumor tissue, which can contribute to assessing response to therapies and improving drug resistance. Attempts to use MR relaxation times measured in cancer cell cultures help assess the biological changes of the applied drug therapy. Relating the results of relaxation times to the results of abundance and viability will allow assessment of the effect of Trastuzumab on viability in cell cultures without interference.