B, N dual-doped Graphene/Au@Pt nanomaterials as sensor for determination of aflatoxin B1

Abstract

In this study, B, N dual-doped graphene/Au@Pt nanomaterials (BN-G/Au@Pt NRs) was prepared by high pressure hydrothermal approach with the mass ratios of graphemes oxide (GO), urea (CH4N2O) and B2O3 were 1:25:30. BN-G/Au@Pt NRs have plenty of monolayer nanosheets with large-area and maintain the characteristic three dimension porous structure of grapheme. More importantly, the as-synthesized BN-G/Au@Pt NRs present advantageous biocompatibility and multivalent affinity interactions with anti-AFB1, which can amplify immunoreactions. Therefore, the designed immunosensor of BN-G/Au@Pt NRs/anti-AFB1/BSA/AFB1/GCE can be used to determinate AFB1 under optimal condition and exhibit excellent sensitivity with a wide linear range from 0.025 ng·mL-1 to 25.00 ng·mL-1 and low the detection limits of 0.017 ng·mL-1. Moreover, the designed BN-G/Au@Pt NRs/anti-AFB1/BSA/AFB1/GCE immunosensor was successfully applied for AFB1 determination in rice samples with acceptable recovery range of 98.0-101.1 %, which demonstrated that the proposed method could be a suitable candidate for AFB1 detection in food industries.