Protocol establishment for micro propagation and in vitro callus regeneration of Maulavi kachu (Xanthosoma sagittifolium L Schott.) from cormel axillary bud meristem

Abstract

The effects of various growth regulators on organogenesis from cormel axillary bud meristems of cultivated species of Maulavi kachu (Xanthosoma sagittifolium (L). Schott. were investigated. Highest percentage (65%) direct regeneration takes place within 25 days of culture but significant number of shoot per plant and length of shoot did not found significantly. Highest percentage (90%) calluses were initiated from axillary buds on MS medium supplemented with 1 mg L-1 BAP+2.0 mg L-1 NAA. Light green, greenish friable or semi friable calluses were found. The highest fresh weight of callus was 11.60 g. These calluses were cultured into MS media containing with 3 mg L-1 Kn + 2 mg L-1 NAA to induce plantlets regeneration. Highest percentage (86%) regeneration was observed with single or aggregated green shoot buds. The average number of green shoot, daughter buds and plantlets were 2.60 and average length of shoots were 7.0 cm with the same medium. Highest percentage of root induction and proliferation were also found into media containing 0.4 mg L-1 IAA and number of roots and root length of regenerated plantlets were 7.90 No. and 6.90 cm, respectively. Plantiets were then established with normal and natural fertile soil on plastic pot showed best performance in acclimatization. © 2007 Academic Journals Inc.