Development of a Highly Boron Tolerant Pseudomonas sp.−Fe-MOF Bioanode

Abstract

In this work, a bioanode which was based on carbon felt electrode (CFE) that contained highly boron tolerant bacterium Pseudomonas sp. isolated from a boron mine (Pseudomonas isolate BC4B) and iron-metal organic framework (Fe-MOF) was developed. To the best of our knowledge, this is the first study in which a highly boron tolerant bacterium, Pseudomonas isolate BC4B was investigated as a bioanode material. It was observed that Fe-MOF increased the signal significantly by behaving as a catalyst. During the study, the optimization of Fe-MOF amount, bacteria amount and substrate concentration were done. Also, the effect of different substrate amounts on the bioanode electrochemical signal was investigated. Consequently, the highest current density value was obtained with 5 mM substrate amount. Meanwhile, in order to investigate the reproducibility of developed bioanode, relative standard deviation value was calculated and found as 1.39 % (n : 3) for 4 mM concentration of H3BO3.