SIMULTANEOUS LC-MS/MS QUANTIFICATION METHOD FOR REMOGLIFLOZIN ETABONATE AND METFORMIN IN PLASMA SAMPLES: APPLICATION OF KINETIC STUDY IN RABBITS

Abstract

A new accurate LC–MS/MS methodology was needed for the simultaneous quantitation of remogliflozin etabonate and metformin in plasma samples. Chromatography was executed with BDS-Hyoersil, C18 (50mm×4.60mm, 5.0µ) reverse phasic analytical stationary system with movable solvent comprising of 0.10% HCOOH, methyl alcohol, and acetonitrile in the fraction of 10:45:45. Multiple reaction monitoring exhibits ionic conversions at m/e 130.14 →85.08, m/e 523.26 → 271.18 and 586.19 → 57.07 for Metformin (MTF), Remogliflozin etabonate (RME), Fosamprenavir internal standard (IS) correspondingly. Method recovery outcomes were not less than 90%, and findings of accuracy values were within the relative error of ±6.0%. MTF has a mean Tmax of 5.62 ± 0.22. AUC0®last was 374.86 ± 9.889 and Cmax was 1.89 ± 1.23 for the sample. RME has a mean Tmax of 52.99 ± 0.12. AUC0®last was 265.83± 9.81 and Cmax was 41.13 ± 0.97 for the sample.