Qualitative and Quantitative Estimation of Crown Gall Inhibitory Activity of Curcumin, Hydroalcoholic Extract of Curcuma aromatica Salisb and Curcuma zedoaria (Christm.) Roscoe

Abstract

Crown gall is a neoplastic disease characterized by the transformation of normal plant cells into autonomous tumor cells in a short period of time in more than 60 families of dicots and many gymnosperms. This paper deals with the comparison of crown gall inhibitory activity of Curcumin, a hydroalcoholic extract of Curcuma aromatica and Curcuma zedoaria both qualitative and quantitative. Qualitative assay results showed that the hydroalcoholic extract of Curcuma aromatica, Curcuma zedoaria, and Curcumin found to possess crown gall inhibitory activity which was found to increase the concentration of the drug. Among the three tested compound curcumin showed good crown gall inhibitory activity from the concentration 500 μg/ml to 1000 μg/ml. In the quantitative assay, all the three tested compounds showed a good percentage of inhibition which was found to be dose-dependent. Among the three compound curcumin showed 56.13% of tumor inhibition at the concentration of 1000 μg/ml, whereas the hydroalcoholic extract of Curcuma aromatica and Curcuma zedoaria showed 29.59 % and 23.46% inhibition of tumor. The less crown gall inhibitory activity of a hydroalcoholic extract of Curcuma aromatica and Curcuma zedoaria might be due to the lower concentration of curcumin.