Focal brain trauma in the cryogenic lesion model in mice

11Citations
Citations of this article
21Readers
Mendeley users who have this article in their library.

Abstract

The method to induce unilateral cryogenic lesions was first described in 1958 by Klatzo. We describe here an adaptation of this model that allows reliable measurement of lesion volume and vasogenic edema by 2, 3, 5-triphenyltetrazolium chloride-staining and Evans blue extravasation in mice. A copper or aluminium cylinder with a tip diameter of 2.5 mm is cooled with liquid nitrogen and placed on the exposed skull bone over the parietal cortex (coordinates from bregma: 1.5 mm posterior, 1.5 mm lateral). The tip diameter and the contact time between the tip and the parietal skull determine the extent of cryolesion. Due to an early damage of the blood brain barrier, the cryogenic cortical injury is characterized by vasogenic edema, marked brain swelling, and inflammation. The lesion grows during the first 24 hours, a process involving complex interactions between endothelial cells, immune cells, cerebral blood flow, and the intracranial pressure. These contribute substantially to the damage from the initial injury. The major advantage of the cryogenic lesion model is the circumscribed and highly reproducible lesion size and location. © 2012 Raslan et al; licensee BioMed Central Ltd.

Cite

CITATION STYLE

APA

Raslan, F., Albert-Weißenberger, C., Ernestus, R. I., Kleinschnitz, C., & Sirén, A. L. (2012, April 5). Focal brain trauma in the cryogenic lesion model in mice. Experimental and Translational Stroke Medicine. https://doi.org/10.1186/2040-7378-4-6

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free