Effects of β-cyclodextrin-dextran polymer on stability properties of trypsin

Abstract

Dextran modified with the mono-6-pentylenediamino-6-deoxy-β-cyclodextrin derivative was evaluated as a thermoprotectant additive for trypsin. The optimum temperature for trypsin activity was increased by 7°C in the presence of this polymer. The enzyme thermostability was increased from 48.5 to 64°C over 10 min of incubation, and the activation free energy of thermoinactivation at 50°C was increased by 4.1 kJ/mol in the presence of the additive. Trypsin was 6-fold more resistant to autolytic inactivation at alkaline pH in the presence of the polymer. © 2003 Wiley Periodicals, Inc.