Low-intensity pulsed ultrasound induced enhanced adipogenesis of adipose-derived stem cells

Abstract

Objective: The aim of this study was to investigate effects of low-intensity pulsed ultrasound (LIPUS) on differentiation of adipose-derived stem cells (ASCs), in vitro. Materials and methods: Murine ASCs were treated with LIPUS for either three or five days, immediately after adipogenic induction, or delayed for 2 days. Expression of adipogenic genes PPAR-γ1, and APN, was examined by real-time PCR. Immunofluorescence (IF) staining was performed to test for PPAR-γ at the protein level. Results: Our data revealed that specific patterns of LIPUS up-regulated levels of both PPAR-γ1 and APN mRNA, and PPAR-γ protein. Conclusions: In culture medium containing adipogenic reagents, LIPUS enhanced ASC adipogenesis. © 2013 Blackwell Publishing Ltd.