Relationship between development of endosperm transfer cells and grain mass in maize

Abstract

The most basal endosperm cells of maize (Zea mays L.) began differentiating into transfer cells in 10 days after pollination (DAP). The thickening and ingrowths forming in the transfer cell wall were slow during 10 and 15 DAP. There were many vesicles, silky and string ball objects in cytoplasm, and the number of mitochondria and rough endoplasm reticulum increased. After 15 DAP, the wall thickening and ingrowths forming in the transfer cells sped up. By 20 DAP, the transfer cell zone had developed, there appeared 65 - 70 rows of cells in width and 3 - 4 layers of cell in depth, the obvious cell wall ingrowths presented strong positive reaction with periodic acid Schiff's reagent. After 20 DAP, no significant change appeared in the shape and structure of the transfer cells, and the transfer cells entered function stage. In the mature kernels (53 DAP), the most basal transfer cells were filled with ingrowths, however, dense cytoplasm was also found in these cells. The nuclei had quite irregular shapes in these cells. Some transfer cells contained black grains and crystals. A black layer formed in the pericarp tissue adjacent to the transfer cell zone. Full development of endosperm transfer cells was important for reduction of kernel abortion and increase of kernel mass.