Development of a fluorescent quantification strip assay for the detection of lead

Abstract

Lead (Pb) is a toxic metal that can accumulate in the body and negatively impact human health. In this study, we prepared a highly sensitive and specific monoclonal antibody, 2C3, that can recognize Pb(II) with an IC50 and linear range of 0.5 ng/mL and 0.08–1.86 ng/mL, respectively. Additionally, based on this antibody we developed a fluorescent test strip, which can be used for both qualitative and quantitative detection of Pb(II) in brown rice with a detection range of 4.67–47.8 ng/mL. Therefore, the method we developed has high sensitivity suitable for the detection of Pb(II) in brown rice.