Molecular re-identification of Stemphylium lycopersici and Stemphylium solani isolates deposited in NCBI GenBank and morphological characteristics of Malaysian isolates

Abstract

Some Stemphylium isolates reported from Malaysia as well as other regions of the world have been identified by morphology and blast analysis of the ITS region only, and eventually deposited in NCBI GenBank. To our knowledge, some of these isolates have been misidentified. The re-examination of identity of all Stemphylium isolates reported from Malaysia using morphological and phylogenetic analyses of the ITS and gpd, as well as phylogenetic analyses of all isolates of S. lycopersici and S. solani available in NCBI GenBank from other regions of the world indicated that a remarkable number of isolates deposited in NCBI GenBank under S. lycopersici and S. solani species were misidentified.