Quantitative Estimation of Gallic Acid and Rutin of Non-Gilled mushrooms Collected from North-Western Himalayas by HPTLC

Abstract

To carry out the quantitative estimation of Gallic acid and Rutin from the methanolic extract of four non-gilled mushrooms by high-performance thin layer chromatography (HPTLC) method. Material and method: Four non-gilled mushrooms (Auricularia auricula-judae, Auriuclaria polytricha, Ganoderma lucidum and Trametes elegans) were collected from North Western Himalayas during the rainy season. Stationary phase for this study was TLC aluminium sheets precoated with silica gel 60 F254 and mobile phase was Toluene: Ethyl acetate: Formic acid (3:6:1v/v) and Chloroform: Ethyl acetate: Methanol: Formic acid (3.5:5:0.5:1 v/v) for Gallic acid and Rutin respectively.The detection and quantification of Gallic acid and Rutin were performed at 280 nm and 254 nm respectively. Results: Rf value of Gallic acid and Rutin were recorded to be (0.43) and (0.70) respectively. Both HPTLC peak areas for standards Gallic acid (6467.8) and Rutin (16592.4) were compared with corresponding peak areas of four non-gilled mushroom extract and the amount of Gallic acid was calculated. All the four non-gilled mushrooms extracts showed the good concentration of Gallic acid and Rutin. But the amount of Gallic acid was higher in comparison to Rutin. Conclusion: This study results showed that the four non-gilled mushrooms extracts are potential sources of antioxidant activity.