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Use of the β-lactamase inhibitor clavulanic acid in the isolation of auxotrophic mutants of Bacillus licheniformis

Journal of Bacteriology (1984) 159(2) 803-804
DOI: 10.1128/jb.159.2.803-804.1984
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Abstract

The isolation of auxotrophic mutants of Bacillus licheniformis, a microbe containing constitutive β-lactamase activity, was found to be facilitated by the addition of clavulanic acid and cefotaxime during enrichment.

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Fields, P. I., Schreier, H. J., Saha, A. L., & Bernlohr, R. W. (1984). Use of the β-lactamase inhibitor clavulanic acid in the isolation of auxotrophic mutants of Bacillus licheniformis. Journal of Bacteriology, 159(2), 803–804. https://doi.org/10.1128/jb.159.2.803-804.1984

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