Angiogenesis system, as a part of endothelial dysfunction in patients with diabetes mellitus type 2: relationship with obesity

Abstract

Aim. To investigate parameters of angiogenesis system in patients with diabetes mellitus and their relationship with obesity. Materials and methods. 104 patients with diabetes mellitus type 2 were included in the study. Patients were divided in 2 groups: Obesity+ (body mass index ≥30 kg/m2; n=63) and Obesity- (body mass index <30 kg/m2; n=41). In all patients was performed clinico-diagnostical examination. mRNA expression levels of vascular endothelial growth factor (VEGF), its receptors flt-1 (fms-like tyrosine kinase 1), KDR (human kinase insert domain receptor) were determined in blood mononuclear cells. Results. There were no statistically significant differences in investigated parameters between study groups. mRNA expression level of VEGF was slightly lower in men compared to women: 0.19 (0.14; 0.32) vs 0.28 (0.12; 0.4) respectively, р=0.2236. MRNA expression level of flt-1 was lower in men compared to women: 0.14 (0.04; 0.3) vs 0.25 (0.12; 0.38), р=0.0321 (statistically significant). We found statistically significant correlations of mRNA expression level of VEGF with mRNA expression level of flt-1 and KDR. Also we found strong positive correlations of BMI and mRNA expression levels VEGF, flt-1, KDR (r=0.86107, r=0.86125, r=0.86112, respectively, p<0.001). Conclusion. Results of the study displayed relationship of obesity and angiogenesis system condition in patients with diabetes mellitus type 2. Further investigations are perspective for the future as a way to new therapeutical approach of obesity and its complications treatment.