Green synthesis of zinc oxide nanoparticles using Zingiber officinale root extract and their applications in glucose biosensor

Abstract

Green synthesis of nanoparticles via plant extracts has become an important research field in nanotechnology. In the present study, a novel amperometric glucose biosensor based on the green synthesized zinc oxide (ZnO) nanoparticles by using Zingiber officinale root was fabricated. Glucose oxidase (GOx) was immobilized onto the ZnO-modified carbon paste electrode (CPE) via cross-linking with glutaraldehyde. The prepared biosensor (GOx-ZnO/CPE) exhibited a good electrocatalytic ability to the determination of glucose. The biosensor also showed a low detection limit (14.7 μM), a rapid response (less than 1 second), high sensitivity (15.98 µA mM−1 cm−2), and higher biological affinity (the Michaelis−Menten constant was estimated as 0.99 mM). Moreover, the prepared biosensor exhibited good anti-interference capability in relation to ascorbic acid (AA) and uric acid (UA). These results demonstrated that a simple and a cost effectiveness biosensor was fabricated for the determination of glucose.