The microbial metabolism of C1 compounds. The cytochromes of Pseudomonas AM1

Abstract

Pseudomonas AM1 contains cytochromes a, b and c and more than one CO binding pigment (cytochrome a3, cytochrome c and possibly a cytochrome o). The soluble cytochrome c was purified; its isoelectric point is low and its molecular weight is 20,000. This cytochrome is reduced in whole bacteria by all oxidizable substrates at rates determined by the primary dehydrogenases. A mutant lacking cytochrome c oxidizes all substrates except methanol, ethanol and methylamine; these no longer support growth. The role of cytochrome c in electron transport in Pseudomonas AM1 is discussed.