FRO GENE PROFILE ANALYSIS FOR CHARACTERIZATION OF IRON STRESS-TOLERANT GENOTYPES IN SWAMP RICE (ORYZA SATIVA L.)

Abstract

Based on the Central Statistics Agency, Indonesia, rice (Oryza sativa L.) production decreased by 2.63 million tons (7.75%) in 2019 compared to 2018, and that could refer to the decrease in rice-growing areas. Therefore, the rice crop area requires enhancement by the utilization of suboptimal tidal swamp lands. However, the heavy iron content in swamp lands is a limiting factor to rice growth. The study aimed to select the iron stress-tolerant rice genotypes by using the effective SSR markers. The arrangement of treatments was a factorial (4×4) completely randomized design with six replications. The first factor was four rice strains (Siam Saba, Siam Tanggung, lNPARI 34, and Ciherang), and the second factor was two iron concentrations (0 and 1,600 ppm) of sulfate heptahydrate solution. The ISC analysis showed the rice genotype Siam Saba appeared to be tolerant to Fe2SO4 stress (1,600 ppm). The SSR marker amplification revealed the primers RM8213, RM252, and RM335 proved more informative and can be effective for genetic studies.