IN VITRO ANTI-ARTHRITIC ACTIVITY OF DIFFERENT EXTRACTS OF CASCABELA THEVETIA (THEVETIA PERUVIANA ) LEAVES

Abstract

Cascabela thevetia (Thevetia peruviana) giant milkweed, is known for its pharmacological importance for centuries. The chemical constituents of Thevetia peruviana leaves are amyrin, amyrin acetate, ?-sitosterol, urosolic acid, cardenolides, calotropin. The anti-arthritic activity of Thevetia peruviana leaves in comparison with Diclofenac was evaluated through in-vitro studies. In-vitro studies revealed anti-arthritic effects (p< 0.05) of crude extracts of aqueous, methanolic, ethanolic and chloroform of Thevetia peruviana leaves on bovine serum albumin as evident for % inhibition protein denaturation. Different extracts of Thevetia peruviana leaves were found to possess in-vitro antiarthritic activity against BSA, using diclofenac as the reference standard which are Dose-dependent (100µg/ml, 250µg/ml, 500µg/ml). Chloroform extract has shown better activity comparing the other extracts.