Optimization of Reaction Parameters for Silver Nanoparticles Synthesis from Fusarium Oxysporum and Determination of Silver Nanoparticles Concentration

Abstract

A number of physical and chemical method available for the production of silver nanoparticles however these methods are quite costly and make use of poisonous chemicals. Thus use of biological organism as bionanofactories offers a clean and cost effective alternative process for the fabrication of silver nanoparticles. Extracellular synthesis of silver nanocrystals from Fusarium oxysporum was accomplished. Data obtained from Ultra violet visible spectrometry was used to calculate the concentration of siver nanoparticles at optimum conditions. The optimum conditions where the concentration of silver nanoaprticles were maximum was found to be 20 g of fungal biomass incubated at 40°C at pH 8.0 using substrate concentration of 2 mM.