A simple and rapid reverse-phase high-performance liquid chromatographic method for the determination of propineb and tricyclazole pesticides from the wettable powder formulation

Abstract

A simple and rapid reverse-phase high-performance liquid chromatographic method was used for the determination of propineb and tricyclazole by pre-column derivatization with ethyl iodide. The factors affecting the derivatization reaction and separation conditions were carefully evaluated and optimized. The optimum derivatization reaction conditions were as follows: reaction time = 15 min, reaction temperature = 40 °C, concentration of ethylenediaminetetraacetic acid solution = 30 mM, pH of ethylenediaminetetraacetic acid solution = 10 and volume of ethyl iodide = 0.5 mL. The method was validated as per the Directorate-General Health and Consumer Protection and International Conference on Harmonization guidelines concerning system suitability, specificity, linearity, precision, accuracy, robustness, limit of detection and limit of quantification. The validated method was successfully applied to the commercially available pesticide formulations, yielding excellent and precise results.