Comparison of inhibitory activity against the α-glucosidase enzymes in the extracts and fractions from leaves of the Garcinia kydia Roxburgh

Abstract

Objective: Garcinia kydia Roxb. is a species of the genus Garcinia, is based chemotaxonomic has various bioactive compounds that have been isolated by a variety of pharmacological activities, one of the activities that are being developed that inhibition of α-glucosidase. However, α-glucosidase inhibitory activity in the extracts and fraction from leaves of the G. kydia Roxb. has not been reported. In this study, seeks to evaluate of α-glucosidase inhibitory activity against extracts and fractions of potentially. Methods: The α-glucosidase inhibitory activity test conducted by in vitro using the enzymatic reaction is measured of quantity with a microplate reader and identifies the compound from the active fraction with normal-phase thin layer chromatography. Results: The ethyl acetate and methanol extract have the potential to inhibit the α-glucosidase with the percent inhibition at a concentration of 500 µg/ml of 83% and 59%, respectively. The active fraction of the ethyl acetate extracts (FEA8) with percentage inhibition at concentrations of 100 µg/ml and inhibitory concentration (IC50) values of 80% and 2.79 μg/ml, respectively, and active fraction of the methanol extracts (FMT3) with percent inhibition at concentrations of 100 µg/ml and IC50 values of 71% and 8.43 μg/ml, respectively. Conclusion: G. kydia Roxb. evident has the potential to inhibit the α-glucosidase. Flavonoid and phenolic compounds that suspected of acts as α-glucosidase inhibitory activity. Thus, the research will continue the process of isolating the active compound so that it can be developed as natural therapeutic agents in the control of glucose.