Spleen cells from primed C57BL/6 (B6) and BALB/c mice generate strong in vitro cytotoxic T lymphocyte (CTL) responses against Sendai virus. In B6 (H-2(b)) mice, this CTL response is restricted by the H-2K(b) molecule, and in BALB/c (H-2(d)) mice by both H-2K(d) and H-2D(d) gene products. Two mutants of H-2K(d), dm4 and dm5, did not differ from the strain of origin, BALB/c, in the magnitude of the Sendai-specific CTL response. Each of nine H-2K(b) mutant mouse strains, however, showed a marked difference in the anti-Sendai CTL response when compared with the strain of origin, B6; bm5, bm6, bm7, bm16, bm17, and bm8 were intermediate responders, bm3 and bm11 were low responders, and bm1 was a nonresponder. B6 anti-Sendai CTL lysed virus-infected (V+) bm5, bm6, bm7, bm16, and bm17 target cells strongly, but less well than B6V+ blasts, whereas bm3V+ and bm8V+ target cells were weakly lysed, and bm11V+ and bm1V+ blasts not lysed at all. In the reverse direction, H-2K(b) mutant anti-Sendai CTL lysed B6V+ target cells to the same extent as syngeneic H-2K(b) mutant V+ targets. These data indicate that the H-2K(b) mutants have lost Sendai CTL restriction specificities without apparent gain of new such specificities. The sequences of relatedness of the mutants with B6 for Sendai CTL restriction specificities and magnitude of Sendai-specific CTL response strongly correspond with the sequence of relatedness among H-2K(b) mutants and B6 based on sharing of CTL allospecificities found previously. In contrast to the inability of bm1 mice to generate Sendai-specific CTL, they showed normal antibody and T cell proliferation responses to Sendai virus. B6/bm1F1 mice were CTL responders after restimulation in vitro with B6V+ or F1V+ spleen cells, but were nonresponders after stimulation with bm1V+ spleen cells. The combined data indicate: 1) class 1 H-2 molecules regulate the Sendai-specific CTL response; 2) CTL restriction specificities are closely related to Ir gene function in this system; and 3) antigenic determinants recognized by allo-CTL and virus-specific CTL are probably identical or are largely overlapping.
CITATION STYLE
de Waal, L. P., Kast, W. M., Melvold, R. W., & Melief, C. J. (1983). Regulation of the cytotoxic T lymphocyte response against Sendai virus analyzed with H-2 mutants. The Journal of Immunology, 130(3), 1090–1096. https://doi.org/10.4049/jimmunol.130.3.1090
Mendeley helps you to discover research relevant for your work.