Relationship of microbial diversity from recycled injection water and high-temperature petroleum reservoirs analyzed by polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE)

Abstract

The microbial communities and diversities in production water from three high-temperature, waterflooded petroleum reservoirs in the same injection area of the Chenghai area of Dagang oil field in China and their recycled injection water sample were characterized by polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) analysis. Results show that microbial sequences mainly belonged to the divisions Proteobacteria, Bacteroidetes, Spirochaetes, Firmicutes and Euryarchaeota. Many mesophilic and thermophilic microorganisms were found in samples of produced water and injection water. Thermoanaerobcter, Desulfotomaculum, Methanolinea, Methanomethylovorans and Geoglobus were detected in all samples. Mesophilic microorganisms found in the samples of produced water, mainly belonged to Brevundimonas, Agrobacterium, Pseudomonas, Sphingomonas, Acinetobacte, Dechloromonas, Clostridium and Methanobacterium. All of them were detected in the injection water samples, except Brevundimonas and Methanobacterium, which indicates that injection exploration may be the main reason why mesophilic bacteria was detected in hightemperature petroleum reservoirs of Dagang. Results suggest that reinjection of water not only introduces enormous mesophilic microbe into high-temperature petroleum reservoirs, but also cause thermophilic microorganisms cross-contamination among wells in the same injection area. © 2011 Academic Journals.