The strong and the stronger: The effects of increasing ozone and nitrogen dioxide concentrations in pollen of different forest species

Abstract

The knowledge of pollen sensitivity and tolerance to stress factors such as air pollution is important for forest sustainability, ensuring the most efficient production with the highest benefits and lowest resource losses. This study intended to evaluate the influence of common air pollutants in four forest trees species, Betula pendula Roth, Corylus avellana L., Acer negundo L. and Quercus robur L., through a comparative analysis at the same experimental conditions. We aimed to investigate the effect that may occur in pollen fertility, protein content, oxidative stress and wall composition after exposure in vitro to ozone and nitrogen dioxide at concentration levels for vegetation protection in Europe. Our results suggest changes in pollen viability, protein content and differential sensitivity related to ROS synthesis, NADPH oxidase activity, as well as in wall composition. The results indicate that NO2 exposure affected more the pollen species studied mostly at the highest concentration exposure. As for ozone, there were less significant differences between samples; however, a different behavior occurs in O3 expositions, where the most influence happens at the legal limit for vegetation protection in Europe. Our study showed that significant pollen functions could be compromised even at common air pollutant’s concentrations.