IC-ELISA and immunochromatographic strip assay based monoclonal antibody for the rapid detection of bisphenol S

Abstract

A highly sensitive and specific monoclonal antibody against bisphenol S (BPS) was prepared. The derived BPS was coupled to keyhole limpet hemocyanin as the immunogen and ovalbumin as the coating antigen. Based on monoclonal antibodies, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was established, and the conditions of action were optimized. The half maximal inhibitory concentration (IC50) of BPS was 0.228 ng/mL and the linear range was 0.064–0.810 ng/mL. In the recovery test for the milk samples, the recovery rates were in the range of 89%–95% and coefficient of variation ranged from 1.0% to 6.0% respectively. In addition, the cut-off value of the immunochromatographic strip detection method in milk samples was 5 ng/mL. Therefore, both methods are suitable for use in milk samples. Furthermore, this immunochromatographic strip detection method is suitable for on-site testing and screening of very large samples.