Discrimination of fluorinated uridine metabolites in N‐417 small cell lung cancer cell extracts via 19F‐ and 31P‐NMR

Abstract

31P‐NMR extract spectra of N‐417 Small Cell Lung Cancer (SCLC) cells cultured with fluorouridine (FUrd) reveal new peaks with chemical shifts in the diphosphodiester and nucleoside triphosphate regions. These peaks were identified as FUTP, FUDP, FUDP‐glucose, FUDP‐glucuronate, FUDP‐GlcNAc, and FUDP‐GalNAc via enzymatic conversion and 19F‐and 31P‐NYR analysis. Distinct 19F chemical shifts were assigned for FUTP, FUDP, and the FUDP‐sugars. Copyright © 1994 Wiley‐Liss, Inc., A Wiley Company