MiR-223 and miR-142 attenuate hematopoietic cell proliferation, and miR-223 positively regulates miR-142 through LMO2 isoforms and CEBP-Β

Abstract

miR-142 and miR-223 have been identified as hematopoietic specific microRNAs. miR-223 has crucial functions in myeloid lineage development. However, the function of miR-142 remains unclear. In this study, we found that both miR-142 and miR-223 attenuated the proliferation of hematopoietic cells, and that miR-223 up-regulated miR-142 expression through the LMO2-L/-S isoforms and CEBP-Β. miR-223 negatively regulated both LMO2-L/-S isoforms and CEBP-Β post-transcriptionally, while CEBP-Β positively regulated the LMO2-L/-S isoforms and both of the LMO2-L/-S isoforms negatively regulated miR-142. These results reveal a novel miR-223CEBP-ΒLMO2miR-142 regulatory pathway, which has pivotal functions in hematopoiesis. © 2010 IBCB, SIBS, CAS All rights reserved.