2,3-Butanedione Monoxime (BDM) Increases Initial Yields and Improves Long-Term Survival of Isolated Cardiac Myocytes

Abstract

When BDM is added to the perfusion solutions used during the preparation of single, enzymatically dispersed mammalian cardiomyocytes, higher yields of calcium-tolerant cells are obtained. As the principal component of a storage solution, BDM also improves the survival of myocytes maintained in cold storage. These data support the hypothesis that BDM can act as a cardioprotective agent under certain conditions. © 1995, PHYSIOLOGICAL SOCIETY OF JAPAN. All rights reserved.