Utilizing of 96% etanol extract activity red leaf (Syzygium myrtifolium walp.) maseration and socletation method as an antidiarrhea at male mice

Abstract

Diarrhea is still one of the most important public health problems because it is the third major contributor to child morbidity and mortality in Indonesia. One of the traditional medicines that can be used to treat diarrhea is red shoots. Red shoot leaves contain secondary metabolites such as alkaloids, flavonoids, chalcones, tannins, and terpenoids which can be used as antidiarrheals. The purpose of this study was to compare the antidiare effects between extracts of maceration and socletation of red shoot leaves and determine the effective dose of both extracts as antidiarrheal in male mice using the intestinal transit method. The treatment in this study consisted of the administration of ethanol extract of shoots of red shoots by maceration at a dose of 1.58 mg / 20 g BW (dose of MI), 4.75 mg / 20g BW (dose of M.II), 6.33 mg / 20g BB (Dosage M.III) and ethanol extract of shoots of red shoots by socletation at a dose of 1.67 mg / 20 g BB (SI dose), 5.04 mg / 20 g BB (Dosage S.II), 6.72 mg / 20 g BB (Dose S.III), Loperamid HCl as positive control, and 1% CMC suspension as negative control. Observations were made by measuring the ratio of intestinal length traveled through the norit (marker) to the total gut length. The results showed that all dose treatments on maceration and socletation ethanol extracts had antidiareal activity. Different extraction methods give the same effect in giving antidiarrheal effects. Based on Duncan's advanced dose of 6.72 mg / 20 g BW of socletation results is an effective dose as antidiarrheal because it has an effect that is not significantly different than Loperamid HCl.