DNA DAMAGE PROTECTION AND HAEMOLYTIC ACTIVITY OF ISOLONGIFOLENE

Abstract

In this study a Sesquiterpene, Isolongifolene of Murraya koenigii tested for its DNA protecting, genotoxicity and haemolytic activity. DNA Protecting activity was tested against genomic DNA using various concns. of isolongifolene and the band intensity of each concn. was calcd. Genotoxicity was studied using pUC19 with different concns. of isolongifolene and the activity was analyzed using gel electrophoresis. Haemolytic activity was assayed using human erythrocytes and the activity was measured at 247 nm. Result shows that isolongifolene may protect the genomic DNA from radical damage and it does not produce any toxicity to the pUC19. The haemolytic activity isolongifolene shows the IC50 value of 50.42 which does not cause haemolysis. Hence, based on the results we conclude that isolongifolene has good protecting activity which can be used for further pharmaceutical studies. [on SciFinder(R)]