Studies on symbiotic association of mycorrhiza with Burkholderia multivorans for sustainable agriculture

Abstract

This study deals the symbiotic association of Scutellospora nigra an arbuscular mycorrhizal fungus with and without the presence of Burkholderia multivorans with Allium cepa as host plant. The inoculum consisted of AM fungus Scutellospora nigra and the Burkholderia multivorans bacterial isolates were prepared and used for this study. Identification of Burkholderia multivorans based on physical and biochemical characters were not sufficient for species identification. Amplification of 16S rRNA gene was a suitable technique for molecular level identification. The sequence of 16S rRNA gene of Burkholderia multivorans was submitted to NCBI database. Burkholderia multivorans was gave antibiotic activity against the antibiotic such as chloramphenicol and tetracycline. Dual inoculation of Scutellospora nigra and Burkholderia multivorans significantly increased the association of AMF Scutellospora nigra with Allium cepa.