Simultaneous separate detection of low angle and large angle light scattering in an arc lamp‐based flow cytometer

Abstract

A device is described for simultaneous separate detection of the light scattering of cells at low and large scattering angles in an arc lamp‐based flow cytometer with epi‐illumination through an oil immersion microscope objective. Light scattering was measured in a dark field configuration that allows separate detection of light scattering greater than 2° and 15°, respectively. Dual parameter light scattering histograms of a blood cell suspension containing various types of leukocytes were closely similar to that obtained with a commercial laser‐based instrument with light scattering detection at forward and right angles. The sensitivity of the device was sufficient to measure polystyrene particles with 0.25‐μm diameter. A potential application may be differentiation of bacteria. Copyright © 1986 Wiley‐Liss, Inc.