Up-regulation of antioxidant status in chronic renal failure rats treated with mesenchymal stem cells and hematopoietic stem cells

Abstract

Background and Objective: Renal failure patients may benefit from kidney transplantation, but the use of immunosuppressive drugs can cause many complications. Stem cell treatment may be a solution to avoid these complications. The current work was an attempt to participate in investigation of the oxidative status of Chronic Renal Failure (CRF) before and after treatment with pluripotent stem cells. Materials and Methods: Sixty four rats were divided into 8 groups. Group I (GI) rats injected only with doxorubicin to initiate CRF and groups II-VII (GII-GVII) rats injected with doxorubicin and treated with stem cells as the following: GII treated with Mesenchymal Stem Cells (MSCs), GIII treated with MSCs and an immunosuppressor, GIV treated with Hematopoietic Stem Cells (HSCs), GV treated with HSCs and an immunosuppressor and GVI treated with MSCs and HSCs, GVII treated with MSCs, HSCs, growth factors extract and an immunosuppressor. GVIII as a negative control (normal rats treated with PBS). Results: The obtained results showed an increase in the oxidants (MDA) and a decrease in the antioxidant parameters (GSH, SOD and CAT) in GI. These parameters restored their normal values after treatment with stem cells (GII-VII) when compared with the normal group (GVIII). Also, the electrophoresis protein patterns of the stem cells treated rats (GII-VII) were similar to that of the normal group (VIII). Conclusion: It can be concluded that stem cells treatment can regenerate the damaged kidney cells and can correct the disturbance in the antioxidant status.