Method Development of Methotrexate in Phosphate Buffer Solution by UV-Visible Spectroscopy

Abstract

A simple, precise, accurate, cost effective stability indicating UV Spectrophotometric method has been developed for the estimation of Methotrexate shows highest ?max at 303nm. Beers law (linearity response) was found over a concentration range of 2-10 -µg /mL with good correlation coefficient (r2 = 0.9987 and the values of standard deviation were satisfactory low and the recovery studies were close to 100%. The Proposed spectrophotometric method was validated as per the ICH Q1A (R2) guidelines. Hence this method can be safely be employed for the routine quality control analysis of Methotrexate. Nikhil Rajnani | Dr. Nalini Satish Kurup"Method Development of Methotrexate in Phosphate Buffer Solution by UV-Visible Spectroscopy" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-4 , June 2018, URL: http://www.ijtsrd.com/papers/ijtsrd14256.pdf http://www.ijtsrd.com/pharmacy/analytical-chemistry/14256/method-development-of-methotrexate-in-phosphate-buffer-solution-by-uv-visible-spectroscopy/nikhil-rajnani