Effects of membranotropic microfertilizers to grow the mycelium of Lentinula edodes

Abstract

Microfertilizers is an important factor for good production of the mycelium of Lentinula edodes mushrooms. The commercial cultivation of mushrooms depends on the correct adjustment of the components of the nutrient media. Three formulas of nutrient media, including decoctions of oak, oatmeal and potatoes. Most mushrooms grow and function well at a pH close to neutral or light basic. Method of light microscopy. Biotechnological (obtaining and subcultuvating of strain 3667 in vitro), microbiological (obtaining pure mushroom culture, studying the cultural properties of the colonies, determining the hydrogen index (pH) of the nutrient medium), mycological (measurement of growth, growth density and dry weight of mycelium, determination of mycelial radial growth, analysis for the presence of buckles and hyphae), light microscopy and statistical methods were used. Performed experiments showed that acceleration of mycelial growth and the greatest yield of mycelium L. edodes were observed on a nutrient medium that contained microfertilizer «Avatar-1». The performed experiments showed that the acceleration of mycelial growth and the greatest yield of mass of mycelium L. edodes were observed on nutrient media with microfertilizer «Avatar-1». During the experiment, it was found that the maximum overgrowth of the medium by mycelium occurs at 7 days. It has been proved that in the «Avatar-1» environments, there was an increase and consolidation of bifurcated hyphae and buckles. The dependence of growth rate on the type of nutrient medium, the administration of doses of the drug, which effectively influences and reduces the technology of obtaining primary mycelium L. edodes, is demonstrated.