Protective effect of Cartilage-selenium polysaccharide on diethylnitrosamine-induced liver injury in mice and its possible mechanisms

Abstract

To study the protective effects and mechanisms of cartilage-selenium polysaccharide on liver injury caused by diethylnitrosamine DEN in mice. During the entire experimental period, to analyze liver injury indexes, the liver was greatly injured by DEN induced and completely prevented by low and high cartilage-selenium polysaccharide. GSH-Px and SOD activities and quantity of mRNA of GSH-Px and SOD were significantly higher to decrease MDA content and -OH and O2 scavenging. Cartilage-selenium polysaccharide was evidently reduced quantity of collagen type I and mRNA of α-SMA and HSP47 compared to model group by RT-PCT detecting. Cartilage-selenium polysaccharide had protective effect on liver injury induced by DEN in mice, and its mechanisms were related to free radical scavenging, increasing SOD and GSH-px activities, enhancing quantity of mRNA of GSH-Px and SOD, decreasing quantity of collagen type I and mRNA of a-SMA and HSP47 and inhibiting the production of proinflammatory mediators.