The effect of Clove Stem Oil (Oleum caryophylli) on the growth of Escherichia coli isolated from native chicken, cattle and pig

Abstract

The objective of this study was to examine the effects of Clove Stem Oil (Oleum caryophylli) or CSO extracts on the growth of Escherichia coli. A 4 x 3 Factorial Design was applied in this study. The first factor was 4 types of E. coli isolates that collected from native chicken (C), young cattle (A), piglet (B1) and pig (B2), while the second factor was 3 concentrations of CSO extract, that was 50.00, 25.00 and 12.50 percents. Fifteen micro liters of CSO extract was dropped in sterile paper disks. These disk were laid on the MEU agar media previously inoculated with each of the four isolates and were incubated overnight at 37°C. The bacterial growth inhibition zones were observed and measured. The results demonstrated that the higher concentration of the CSO extracts, the higher bacterial growth inhibition effects obtained. Fhe growth inhibition effects of the CSO extracts on E. coli isolates C, A, B1 and B2 were significantly different at P<0.05. It could be concluded that the CSO extracts were effective to control the E. coli growth.