Production of long-chain levan by a sacc insertional mutant from bacillus subtilis 327UH

Abstract

A hyper extracellular protein producer, Bacillus subtilis 327UH, produced large amounts of levan in a medium containing 20% sucrose, and the yield of levan after 10 hours was more than 60%, when based on the fructose amount of sucrose. After transformation of 327UH with a levanase-deficient 168SC (sacC::Cmr) chromosomal DNA, a Cmr transformant 327UHSC (sacC::Cmr degSU(Hy)) produced 3 times longer levan than that of the wild type. © 2002 by Japan Society for Bioscience, Biotechnology, and Agrochemistry.