Fluorescence imaging under background light with a self‐reset complementary metal–oxide–semiconductor image sensor

Abstract

The authors propose and demonstrate the fluorescence imaging of green fluorescence protein (GFP) expressed in a brain slice with a self‐reset complementary metal–oxide–semiconductor image sensor under background light. By using a self‐reset function to avoid pixel saturation, the weak fluorescence of GFP was successfully observed, even under background light. The result suggests that the sensor can be applied to in vivo imaging of laboratory animals during light–dark cycles, so that they can observe the different responses to bright and dark environments.