Biomimetically synthesized ZnO nanoparticles attain potent antibacterial activity against less susceptible: S. aureus skin infection in experimental animals

Abstract

In the present study, we explored the antibacterial potential of ZnO-nanoparticles that were biomimetically synthesized employing conditioned culture supernatant of Staphylococcus aureus (S. aureus). The in situ synthesized ZnO-NPs were characterized employing various biophysical techniques including UV-vis spectroscopy, electron microscopy, DLS and XRD analysis etc. Electron microscopic studies revealed the disintegration of bacterial cell wall upon interaction with synthesized ZnO-NPs. Various employed tests provided evidence that synthesized ZnO-NPs effectively inhibited less susceptible Gram-positive and Gram-negative bacterial isolates. Moreover, the synthesized ZnO-NPs inhibited the biofilm synthesis of tested microbes in vitro. Finally, the biomimetically synthesized ZnO-NPs successfully treated the S. aureus mediated experimental skin infection in BALB/c mice as well.