A technique comparison of isolation of lipolytic bacteria

Abstract

Three different agar diffusion screening methods, employing two growth media, standard plate count agar (SPCA) and 1% peptone enriched trypticase soy agar (TSA), were used to isolate psychrotrophic bacteria from raw milk which produce lipase. Clarified butterfat and nile blue sulfate served as the substrate and dye, respectively, in all methods. Although a double-overlay method yielded slightly greater numbers of lipolytic bacteria than a single-layered method with comparable media, better visual clarity was achieved with the single-layered method. Peptone enriched TSA medium supported better growth of lipolytic bacteria than SPCA.