Single‐cell third‐generation sequencing‐based multi‐omics uncovers gene expression changes governed by ecDNA and structural variants in cancer cells

Abstract

scGTP-seq is a third-generation sequencing (TGS) platform-based single-cell multi-omics method that enables simultaneous sequencing of the genome and transcriptome. This method can efficiently detect ecDNA and SVs and determine how these genomic variations impact transcriptomes at the single-cell level. It is a powerful tool to investigate the regulatory mechanisms of ecDNA and SVs across diverse cell types and clinical tumour samples. Clin. Transl. Med. 2023;13:e1351. wileyonlinelibrary.com/journal/ctm2 https://doi.