Interaction between DNA and an Escherichia coli protein ω

Abstract

An E. coli protein, designated ω, has been purified at least 1000-fold. Treatment of a eovalently closed DNA duplex containing negative superhelical turns with ω results in the loss of most of the superhelical turns. The loss of superhelical turns follows a gradual course rather than a one-hit mechanism. This reaction does not require a cofactor. No other change in the physical properties of the DNA could be detected. The DNA remains covalently closed. Its ultraviolet absorption spectrum, circular dichroism, buoyant density in CsCl, sedimentation properties in neutral media containing varying amounts of ethidium and in an alkaline medium, and its susceptibility toward Neurospora endonuclease, are not significantly different from an untreated DNA containing the same number of superhelical turns. Thus it appears that ω is capable of introducing a "swivel" reversibly into a DNA. A plausible mechanism is postulated. © 1971.