Preliminary study on the effects of carbon dioxide and nitrogen pneumoperitoneums on endometriotic lesions

Abstract

Objective: To determine the effects of carbon dioxide (CO2) and nitrogen (N2) pneumoperitoneums on endometriosis (EMs) lesions. Methods: Female Wistar rats were randomized into the following 3 groups: CO 2 (N = 20), N2 (N = 22) and air pneumoperitoneums (N = 9). After 5 weeks of establishment models, do the pneumoperitoneums. Then measure the size of EMs lesions and the related factors of serum and tissue after 1, 2, and 4 weeks of pneumoperitoneums. Results: (1) One week after the pneumoperitoneum was established, the EMs lesions in the CO2 group were largest in volume, whereas at 4 weeks the EMs lesions in the CO2 group were smaller than the N2 group. (2) The level of ICAM-1 and TIMP-2 of serum in CO2 and N2 group after 2 weeks of pneumoperitoneum were higher than air group. (3) The expression of CD44v6, ICAM-1, MMP-2 and VEGF of tissue in CO2 and N2 group after 1, 2 and 4 weeks of pneumoperitoneum were lower than air group, TIMP-2 and ENS were higher than air group. Conclusion: After a CO2 pneumoperitoneum, EMs lesions were reduced in volume, suggesting an inhibitory effect on EMs lesions. © The Author(s) 2012.