Characterisation and mobilisation of IncA/C plasmid-mediated antibiotic resistance in Edwardsiella ictaluri

Abstract

Objectives: Edwardsiella ictaluri is an important pathogen in farmed raised catfish. Recently, we showed that resistance to tetracycline and florfenicol in the E. ictaluri MS-17–156 strain isolated from channel catfish was facilitated by acquisition of a 135 kb plasmid (named pEIMS-171561). Methods: We described the genetic structure of pEIMS-171561. Plasmid copy number and stability within E. ictaluri strain MS-17–156 was determined. We also investigated the in vitro and in vivo transferability of pEIMS-171561 using catfish as a model for in vivo transfer. Results: pEIMS-171561 belonged to the IncA/C group and contained florfenicol efflux major facilitator superfamily (MFS) (floR), sulfonamides (sul2), and tetracycline efflux MFS (tetD) genes. The plasmid contained two conjugative transfer-associated regions and encoded six transposases and insertion sequences. In vitro conjugation experiments demonstrated that the IncA/C plasmid can transfer from E. ictaluri to Escherichia coli. The plasmid was stable in E. ictaluri without selection pressure for 33 days. We showed that pEIMS-171561 did not transfer from E. ictaluri MS-17–156 to endogenous microbiota in catfish. Moreover, we could not detect in vivo conjugal transfer of pEIMS-171561 from E. ictaluri to E. coli. Results from real-time PCR revealed upregulation of the floR gene in the intestines of catfish receiving florfenicol-medicated feed, compared with that in catfish receiving unmedicated feed. Conclusion: This study demonstrated that pEIMS-171561 did not disseminate from E. ictaluri to gut microbiota under selective pressure. This result suggests a limited role of the fish microbiota as a reservoir for this plasmid and for the spread of resistance.