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Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatography

Brazilian Archives of Biology and Technology (2005) 48(SPEC. ISS.) 113-117
DOI: 10.1590/s1516-89132005000400014
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Abstract

This paper describes a method for the plasmid DNA purification, which includes an ammonium sulphate precipitation, followed by hydrophobic interaction chromatography (HIC) using Phenyl Sepharose 6 Fast Flow (low sub). The use of HIC took advantage of the more hydrophobic character of single stranded nucleic acid impurities as compared with double-stranded plasmid DNA.

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Moreira, K. A., Diogo, M., Prazeres, D. M., De Lima Filho, J. L., & Figueiredo Porto, A. L. (2005). Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatography. Brazilian Archives of Biology and Technology, 48(SPEC. ISS.), 113–117. https://doi.org/10.1590/s1516-89132005000400014

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