Factors affecting to somatic embryogenesis and plant regeneration from callus and In Vitro Ontogeny of Doritaenopsis taisuco ladylip

Abstract

The efficient plant regeneration protocols were developed for Doritaenopsis Taisuco Ladylip through callus culture and somatic embryogenesis. The embryogenic calli were cultured on half strength Murashige and Skoog (MS) medium supplemented with 0.5 mg·L-1 thidiazuron, 2.5 mg·L-1 2,4-dichlorophenoxyacetic acid, 30g·L-1 sucrose and 3g·L-1 Geirrte for proliferation. Basal salts, carbohydrate of medium obviously affected the somatic embryogenesis of callus. The highest frequency of somatic embryogenesis was obtained when embryogenic calli were cultured on half strength MS medium containing 20g·L-1 maltose or 10g·L-1 trehalose. The protocorm-like bodies well developed into plantlets after changing the basal salts to Hyponex, and carbohydrate to sucrose. The histological processing of somatic embryogenesis from callus was observed with light microscopy, and somatic embryo was clearly origin from single cell.