Prostate protein n-glycosylation profiling by means of DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis

Abstract

DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis allows for accurate profiling of the asparagine-linked (n-) glycosylation patterns, a posttranslational modification present on many soluble and membrane proteins. This technique has been extensively tested to identify n-glycosylation patterns associated with serum proteins. Here we describe the use of DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis to identify the n-glycosylation patterns of prostate proteins in urine.